Natto: Japanese History of a Modern-Day Superfood

 
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Natto is often seen as an up-and-coming new superfood in the West, but in Japan natto has been a staple item in the traditional Japanese diet for centuries. While many elements of typical Japanese cuisine (washoku) like miso soup, sushi, tempura, ramen, sake have become well known western culture, natto is an equally common dish that has largely remained obscure. But food culture can evolve rapidly. For example, remember that only a few decades ago, sushi was bizarre and widely reviled; now it is such a familiar part of the American food landscape.

 
Wide variety of natto sold in Japanese grocery stores

Wide variety of natto sold in Japanese grocery stores

 

In Japan, natto is most commonly consumed as part of a typical everyday breakfast meal. A hearty morning repast of Natto with rice, miso soup and pickled vegetables could be the Japanese equivalent of a Western standard morning plate of eggs, bacon & toast. Many Japanese eat natto on a daily basis, or nearly so. In a typical grocery store in Japan, one may find dozens of varieties and brands of natto on the shelves. Another good metaphor for natto might be ‘yogurt’: a common, protein and nutrient-dense fermented food category with an entire section of refrigerated space devoted to selections.

 

How to Eat Natto the Old School Way

READ MORE »

 

The History of Natto

The precise origins of when natto was invented are uncertain. This is often the case with food origin stories, especially those of fermented foods, which were probably ‘discovered’ by accident in cultures throughout the world. In ancient times before refrigeration was possible, spontaneous wild fermentation of foods coming into contact with environmental microbes led to all sorts of delightful ferments: wine, cheese, beer, sauerkraut, coffee and chocolate to name just a few favorites! Fermentation also provided a method to preserve perishable food from spoilage and extend the lifetime of valuable nutritional resources into the lean winter season

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The samurai story: Minamoto no Yoshīe

The most popular legend dates natto’s discovery to the 1080’s AD during the Gosannen War, when the great Samurai Lord Minamoto no Yoshīe and his army were traveling towards conquests in northern mainland Japan. One night, the warriors took refuge at a country farm and settled down for a simple evening meal of rice and boiled soybeans, when they got word of an enemy approach. They decided to retreat, quickly wrapping up their food in rice straw they had to feed their horses. The straw provided a natural source of soil-dwelling Bacillus subtilis bacteria to initiate fermentation. Running straight through the night on horseback, the bundles of straw-wrapped soybeans were warmed in the heat and moisture of the horses’ sweat. After reaching safety day or two later, they opened the bundles to discover the soybeans fermented and supposedly found them to be both tasty and nourishing. 

Other possible origins

An alternative natto origin story dates an earlier emergence of natto in 7th Century Japan tied to the emergence of Buddhism in Japan. In this theory, Prince Shotoku (574-622 AD) is credited with a similar accidental discovery of natto from a parcel of cooked soybeans wrapped in rice straw. A devout Buddhist scholar, Shotoku is believed to have authored and translated the first Buddhist religious texts in Japanese. Natto eventually became a core protein-rich component of the devotional vegan diet of Zen Buddhist monks, shojin-ryori cuisine. This narrative may also suggest that natto, like so many culinary, cultural and religious ideas, originated in China.  Some historians even trace natto’s origins to similar soybean ferments during the Chinese Zhou Dynasty arriving in Japan in the Yayoi period (300 BC – 300AD)! One thing is for sure, Natto has been a part of Japan’s washoku for a very long time.


Natto’s Popularity in Japan

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Natto’s popularity spread gradually, starting from the region of Mito city in Ibaraki Prefecture, where the legend of Minamoto no Yoshie took place. Today, natto is eaten by many millions of people on a daily basis, and consumption is highest in the eastern and northern parts of Japan. By the 17th Century start of the Edo period, natto was well incorporated into everyday cuisine with walking vendors selling fresh, locally made wara natto in the streets each morning.

In Edo Japan, natto became a nutritious staple food of the working class, typically eaten with rice and pickles or mixed into miso soup (natto-jiru: see our previous blog post for recipe). At first, natto was made and eaten mostly in the cooler fall and winter months when the nattokin cultures seemed to grow best. As better understanding of how to control the nattokin fermentation in warmer weather developed, makers began producing natto year-round.

Mito: the Birthplace of Natto

Approximately half a million tons of natto are made and consumed annually in Japan, a country smaller (though admittedly more crowded) than the state of California. Historically, the Mito region was also the center of natto production, as the area also happened to be where many farmers grew small soybeans best suited for making natto. Today, Mito city identifies as the birthplace of natto and remains the epicenter of natto culture in Japan.

 
Ann with the wara statue in Mito

Ann with the wara statue in Mito

 

In 2017, I made a pilgrimage of sorts to Mito to learn more about the origins of this Japanese food and to visit some shokunin, the oldest artisanal makers of natto still producing with traditional methods rarely seen elsewhere. Small producers may also make seasonal specialty natto products; for example, natto mixed with pickled vegetables or dry natto.

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For example, in Mito one can still buy natto packaged in old-fashioned rice straw bundles (wara) instead of the environmentally unfriendly styrofoam boxes that are the ubiquitous elsewhere. Upon arriving in Mito, I was greeted by a giant metal monument dedicated to natto, in the shape of a natto wara just outside the train station!

Just like in the legends, for centuries natto was actually made with rice straw as the natural source of fermenting Bacillus subtilis bacteria. Bundles of straw (called wara) were wrapped around small amounts of boiled soybeans and incubated in a warm environment to ferment. In rural areas some DYIers still make natto at home using straw wara6, but this method is no longer widely used in commercial natto production because of fermentation variability and potential for food contamination. But in homage to history, a handful of makers still produce limited edition wara natto, usually purchased as a souvenir or gift item.

Japanese Natto Today

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I was fortunate to meet Mito native, natto advocate and entrepreneur Miyashita-San, who brought me to the primary remaining producer of wara, Mito Natto. The basics of natto making are dictated by the biology of the fermentation and are therefore pretty similar everywhere, but each maker adds their own variations in materials and methods to give their products a unique character. It was inspiring to see how every step of the natto process was still done by hand here, including careful packing of the straw wara.

To ensure safe, reliable product fermentation, now all natto commercially produced in Japan is made using purified nattokin starter cultures. Here, already inoculated soybeans filled inside bundles of pre-sterilized wara straw. This natto has a noticeably stronger taste than Mito Natto’s other natto varieties which are packaged in plastic containers. This may be because the wara allow a lot of airflow into the natto, accelerating further growth and fermentation by the nattokin cultures.

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This was the first time I’d come to Japan smuggling samples of my own New York Natto. It was intimating to offer Mito Natto company president Takaboshi-San a taste! First of all, he was quite amazed to see our natto in a glass jar; natto had never been sold this way in Japan. Next, he gave our natto a stretch and was impressed by our strong neba-neba (stretchy, slimy, sticky quality). And finally, we got a thumbs up on flavor from Takahoshi-San too! Phew, I was more than delighted to hear “This is real natto!” from an award-winning, fourth-generation natto maker. We’ve since shared our natto with many other Japanese natto producers at the national natto competition, but that’s another story.

 

Why is Natto so Sticky

READ MORE »

 

In many places, natto rarely appears on menus of Japanese restaurants; this is mostly because natto is primarily a breakfast dish which most venues don’t serve. In Mito, local natto is served with pride at any time of day in so many eating establishments, some venues even specializing in natto-centric cuisine! Before leaving Mito, I enjoyed a lovely “Neba neba” donburi—a rice bowl topped with many ingredients with that beloved sticky, slimy texture that is so revered and believed to convey healthiness in food here.

Neba-neba ingredients in this delicious bento bowl from an unassuming train station lunch counter included: squid, salmon roe, nameko mushroom, seaweed, tororo yam, raw egg yolk and, of course, natto.

Neba-neba ingredients in bento bowl


Natto News

We’re excited to add two new stores to our list of places to find NYrture Natto!

Good Witch Coffee Bar

134 Southside Ave. Hastings-on-Hudson, NY 10706)

Joanne Prisco’s café & pantry, housed right inside the quaint Metro-North Railroad station celebrates women by featuring products from local female makers (thank you!) and supporting feminist community organization. Learn more about her café.

 

Tawara Mart

55 West Main St. Ramsey NJ 07448

Tawara Mart is the first curated Japanese grocery & bakery in NJ, located next door to the Ishii family’s Japanese restaurant, a local favorite since 1981. We’re excited to have our fresh natto among their thoughtful selection which brings an authentic, modern sensibility to break the mold of the standard Asian grocery!


NYrture New York Natto is produced by hand in NYC, fresh and never frozen, to deliver the best quality natto with maximum potential health benefits to you.

We welcome your feedback! Please share your questions & comments below!

Related links on NYrture.com:

NYrture New York Natto is dedicated to providing America access to fresh, premium natto, the best natural source of Vitamin K2, nattokinase & spore probiotics.

Natto & the Science of Vitamin K2

We heard from lots of you that you want to learn more about Vitamin K2, so here is an introduction to this underappreciated nutrient.

Some of you may be familiar with this basic background info, so we’ve also provided opportunities to dig deeper with links to further articles and primary scientific research literature. Others who may come to natto as a source of probiotics or nattokinase may find a whole new reason to love it!

There’s so much to share and more new information about Vitamin K2 being discovered every year, so we’ll take it one bite-size piece at a time! Subscribe to our newsletter for the latest updates, we’ll focus in on one particular health disorder/disease state and its relationship to Vitamin K2, sharing current views and data from recent science. Find our newsletter sign-up form in the footer of this page.

Why is Vitamin K2 important?

Vitamin K2 is a critical dietary micronutrient that is virtually absent in the modern Western diet. Any comprehensive discussion of Vitamin K2 will include mention of Natto, as this unique food is well known to be the absolute richest food source of Vitamin K2.

Vitamin K2 is receiving more and more attention in medical and nutrition circles because of growing evidence that Vitamin K2 may be a pivotal factor in the prevention of osteoporosis as well as cardiovascular & coronary disease, kidney disorders, diabetes and cancer. 

How can all these common chronic diseases be linked to Vitamin K2, a micronutrient that most people haven’t even heard of? Because K Vitamins are critical cofactors for a variety of Vitamin K-dependent proteins (VDKs) in the body, including factors involved in blood clotting but also many others involved in calcium transport as well as insulin regulation, fat deposition, cell proliferation and DNA transcription. 


What is Vitamin K? 

There is a lot of confusing information about Vitamin K out there in the infosphere. Much of this confusion arises from the unclear nomenclature of Vitamin K and all of its different isoforms. Whenever you see information or intake recommendations regarding “Vitamin K”, this usually refers to both K1 and K2 collectively. Since Vitamin K2 is present in such vanishingly small amounts in most foods, unless otherwise specified, “Vitamin K content” practically always means ‘only Vitamin K1’.

Like the B vitamins, the term “Vitamin K” actually refers to a family of related molecules that can be broadly divided between Vitamin K1 (phylloquinone) and Vitamin K2 (menaquinone).

As you can see below, all the Vitamin K family members share a similar chemical structure. They all contain in common a napthoquinone double ring on the left of these drawings attached to varying types of side chains. Although there is some overlap in physiological functions for the various Vitamin K types, Vitamin K2 appears to play little role in blood clotting and instead is best known to function in regulating calcium transport within the body. Many additional physiological functions of K Vitamins are actively being studied.

Vitamin K2 (MKs or menaquinones) can further be subdivided into a family of closely related molecules that differ in the length of their side-chain structure.

The simplest and shortest of the menaquinones is MK-4, extending up to MK-14, though MK-10-14 have been found only at very low levels in some fermented foods. Among the MK isoforms of K2, MK-4 and MK-7 are the best studied and the only two that are available also in supplement form.

Among the MK isoforms of K2, MK-4 and MK-7 are the best studied and the only two that are available also in supplement form. 

In supplements, MK-4 is a synthetic compound. 

MK-7 is still commercially produced by purification from bacterial natto fermentation.

It matters what kind of vitamin K we get. The slightly differing structures of these  Vitamin K molecules affect how they each function and distribute themselves within the body and in the foods they’re found in.  


What foods are good sources of Vitamin K?

We humans need to take in K Vitamins through food. These fat-soluble vitamins are essential nutrients that we cannot make by ourselves. We must rely on other organisms that we ingest to provide them for us. Vitamin K1 and K2 are found in very different food sources. Further, specific types of MK menaquinones also appear in different types of foods.

Food sources of Vitamin K from chrismasterjohnphd.com

Food sources of Vitamin K from chrismasterjohnphd.com

What foods contain Vitamin K1?

Vitamin K1 is only made by plants where it functions in electron transport during plant photosynthesis and thus is found in abundance in green, leafy vegetables. Common dark leafy greens like spinach or collard greens contain upwards of 500 micrograms of K1 per 100g. As long as you are including green vegetables in your diet, Vitamin K1 deficiency is rare; Vitamin K2 is a different story.

What foods contain Vitamin K2?

Most forms of Vitamin K2 can be made only by bacteria, where it functions in electron transport in energy metabolism (analogous to K1’s role in bacteria-derived plant chloroplasts). Thus, Vitamin K2 can be consumed in the form of fermented foods such as natto, and European hard cheeses. But different fermented foods contain wildly differing amounts of Vitamin K2. For example, Natto contains about 15x times more Vitamin K2 as the best cheese sources and ~200x times as much as sauerkraut!

The exception is MK-4, which animals and humans can synthesize in small amounts from dietary Vitamin K1 phylloquinone or menadione. How much MK-4 can be generated has been estimated to range from 5% to 25% of the ingested phylloquinone, but this provides neither the levels nor diversity and distribution of menaquinones needed to fully support all Vitamin K2-dependent functions throughout the body.

This is why measurable amounts of MK-4 can be found in fatty dairy and meat-based foods.  Herbivorous animals like cattle ingest large amounts of plant Vitamin K1 and can convert some of it to MK-4. K vitamins are fat-soluble and therefore accumulate most in fat-rich tissues, organs (like liver, brain) and milk. 

Some cheeses, because they are further fermented with microbes which also produce significant amounts of longer chain forms of MK menaquinones have additional VItamin K2 content. Even among cheeses, there is a lot of variation in how much K2 is present—dependent on the quality of the milk (grass-fed, quality milk will begin with higher K1 & MK-4 content) and the particular strains of microbes used to produce the cheese (different bacterial strains & species vary greatly in their ability to synthesize various MK isoforms). 


How much Vitamin K2 do I need?

The USDA has not yet established a Recommended Daily Allowance (RDA) for Vitamin K. The US Institute of Medicine has set an Adequate Intake (AI) at 90 and 120 micrograms per day for adult women and men. However, this recommendation is based on phylloquinone (K1) and its required levels for proper regulation of blood clotting, as the primary form of Vitamin K in the Western diet. This Vitamin K AI does not address dosage requirements for K2 menaquinones because (a) sufficient food composition analyses of K2 content in Western foods and (b) new science demonstrating the specific importance of K2 menaquinones in calcium regulation, bone & cardiovascular health did not yet exist at the time. 

So long story short: there still is no government-sanctioned intake recommendation specifically for Vitamin K2, but scientists are working on it! For now, medical advocates of Vitamin K2 generally recommend amounts comparable to those established for Vitamin K1 (~100 micrograms or more daily) for healthy adults. For those at risk for bone loss (osteoporosis, osteopenia, arterial calcification, kidney stones) or seeking other Vitamin K2-dependent health benefits, higher amounts may be needed. 

How much natto should you eat to get your daily dose of K2? A single tablespoon of natto contains more than 150 micrograms of Vitamin K2 as MK-7 along with Bacillus subtilis probiotics, nattokinase enzyme in the natural context of a nutritious, protein-rich whole food. Take a look at our Natto Recipes page for ideas on how to eat more natto.


Why is Vitamin K2 Deficiency so prevalent?

There are many factors that contribute to Vitamin K2 deficiency; one of the most important is diet. The modern American food system and typical Western diet have made it more difficult to take in adequate amounts of dietary Vitamin K2.

In the West, major dietary sources of MK-4 include animal-based foods in the form of meat, organ & dairy products. As is true for many nutrients, the MK-4 content of these foods can be highly dependent on exactly how the animals are raised and fed. Pastured and grass-fed animals will naturally take in much more Vitamin K1, a starting material which can be converted to MK-4, from the plant matter they eat. As more and more meat and dairy animals are factory farmed and grain-fed, they lose Vitamin K1 and therefore also Vitamin K2 MK-4 content.

Longer chain menaquinones (e.g. MK-7, 8, 9) are produced naturally by probiotic bacteria (e.g. Bacillus subtilis) and accumulating to significant levels in some fatty fermented foods. Certain cheeses, again ideally made from grass-fed, Vitamin K1-rich milk, have significant levels of higher MK menaquinones made by fermenting bacteria during cheese production. Living, probiotic fermented foods once had a significant presence in culinary traditions of almost every culture around the world. The modern industrially produced diet has limited access to many of these natural menaquinone food sources from our food system.

As you can see from the bar chart below showing the relative Vitamin K1 & K2 contents of top food sources, nothing even comes close to natto!

Natto is the BEST Source of Vitamin K2, particularly for bones

Natto contains far more Vitamin K2 than any other food ever tested. It is also the only significant plant-based, vegan food source of Vitamin K2 available. About 90% of Natto’s K2 is in MK-7 form, the type of menaquinone which has the strongest evidence for bone health benefit.

Numerous observational and interventional clinical studies have shown convincing correlations between dietary Vitamin K2 intake and lower bone fracture rates and higher bone density mass (BDM), particularly in post-menopausal women. Research shows that natto is a great food for bone health.

Precisely how Vitamin K2 helps build healthy bone and prevent bone loss will be the focus of our next science update in a few weeks. We’ll present a summary of current clinical research data showing the remarkable correlations between dietary Vitamin K2 (in the form of food) and bone strength and density.

Read more about in our deep dive about natto & bone health.

Disclaimer: Statements in this article are the summary of our evaluation of scientific literature and have not been evaluated by the FDA. This product is not intended to diagnose, treat or cure disease.


Recommended Reading for a Deeper Dive into Vitamin K2

Vitamin K2 on ChrisMasterjohnPhD.com

Vitamin K2: The Missing Nutrient for Heart and Bone Health by Dr. Dennis Goodman

Vitamin K2 and the Calcium Paradox by Dr. Kate Rheaume-Bleue


NYrture New York Natto is produced by hand in NYC, fresh and never frozen, to deliver the best quality natto with maximum potential health benefits to you.

We welcome your feedback! Please share your questions & comments below!

Related links on NYrture.com:


NYrture New York Natto is dedicated to providing America access to fresh, premium natto, the best natural source of Vitamin K2, nattokinase & spore probiotics.

Life Cycles

In case you missed reading our last blog post (Gaga for Goo), we recently traveled to Boston to geek out and work with scientist friends at Harvard Medical School to investigate the probiotic nature of our natto. Among the many people we have to thank (below, from left to right): David RudnerDana Boyd Joe Davis with Ann Yonetani.

Dr. David Rudner and his lab study Bacillus subtilis (the same bacterial species which ferments natto) because of the interesting biology of its life cycle. They are investigating how these bacteria undergo sporulation, a developmental program which allows them to enter a dormant state in the form of a seed-like spore

Certain species of primitive plants, protozoa, algae, fungi and bacteria can form spores as part of their natural life cycle. Spores are "a unit of asexual reproduction…adapted for dispersal and survival, often for long periods of time, in unfavorable conditions." (2)

Great cartoon/microscopy images of how Bacillus subtilis undergoes the process of sporulation, transitioning from a normally growing bacterial cell (left) through stages of creating a small separate intracellular compartment (second from left) which…

Great cartoon/microscopy images of how Bacillus subtilis undergoes the process of sporulation, transitioning from a normally growing bacterial cell (left) through stages of creating a small separate intracellular compartment (second from left) which then becomes engulfed inside the larger cell (third from left), eventually maturing and being released from the dead cell (right) as a dormant and highly indestructible SPORE. Image by David Rudner, HMS (1)

NYrture New York Natto is created by inoculating hot, pressure-steamed, GMO-free, whole soybeans with spores of Bacillus subtilis (var. natto). Triggered by the heat and rich source of nutrients the beans provide, these spores "wake up" or germinate and begin re-entering a growing phase of their life cycle.   In David's lab, we were able to use tools of scientific inquiry to observe and measure the probiotic bacteria living inside our natto.

Spores don't really look like they do in the video game SPORE (above, left). Rather, Bacillus subtilis spores typically look like this (right); round structures about 1um in diameter, viewed above by confocal microscopy with a fluorescent membrane-staining dye outlining the spore edges (3). We scooped up some NYrture New York Natto "goo", slapped it on glass slides and had a look….

Screenshot from confocal microscope (phase/DAPI stain) of a direct sample from our natto. Not pretty, but a field of thousands of both dead (lighter) and living (dark with halos) Bacillus subtilis cells in our food. Biologists may notice that living…

Screenshot from confocal microscope (phase/DAPI stain) of a direct sample from our natto. Not pretty, but a field of thousands of both dead (lighter) and living (dark with halos) Bacillus subtilis cells in our food. Biologists may notice that living cells of different lengths are seen, indicating that these cells are at various stages of their vegetative life cycle, actively growing & dividing.

We were super excited to see so very many live Bacillus subtilis both actively growing and undergoing sporulation inside our food! This is significant because the vast majority of bacteria (beneficial or not) taken in through food are destroyed during (food storage and) digestion by the extremely acidic stomach environment and/or anaerobic conditions in the gut.  This calls into serious question the actual utility of most probiotic foods/products out there.

But further, under the microscope it looked like at least some of these living cells in our natto were also undergoing sporulation! This was intriguing because if our natto also contains fresh spores (which are nearly indestructible and therefore resistant to digestive assault), these spores are certain to be able to reach the gut where they can germinate and effectively colonize and grow, contributing to human gut health! So, here's what we did to find out...

This (above) petri plate shows a series of serial dilutions of natto "goo" taken directly from a jar of month-old NYrture New York Natto spotted in dots across a line on the agar media near the top edge of the dish; then the plate was held on its side to allow each dilution sample spot of liquid to drip by gravity downwards to form straight lines streaking down the agar surface before being let to grow overnight in a warm incubator. Starting from the left, a 1:1 dilution of natto "goo", followed by serial 10x dilutions (10x, 100x, 1000x, etc…) of the first, left-hand sample.  By diluting the sample down enough orders of magnitude, one can pretty accurately estimate the number of individual cells present in the original sample from the number of colonies (visible spots of cells) that appear the next day.  From this, Alex Meeske in the Rudner lab, gave us an approximation of ~15 million live Bacillus subtilis cells in each milliliter of NYrture natto "goo"(4).  

But now we wanted to know IF and HOW MANY of these cells/colonies came from SPORES also present in the natto "goo"? Here, we can take advantage of the fact that spores are so hardy and resistant to heat by boiling each of the samples for twenty minutes!  No bacterial cells can survive this hot tub, but spores can! So what do we see if we plate the boiled, heat-killed samples in the same manner as above?

We saw that some Bacillus subtilis cells DID GROW from spores present in the boiled, heat-killed samples!  By comparing the number of colonies in this streak (coming from the most concentrated left sample) to those on the previous (unheated samples) plate, we see that it is close to that of the third (100x) dilution. Therefore, we can guestimate that ~1% of the Bacillus subtilis growing from our natto are derived from spores in the food. This translates to about 1.5 million spores per milliliter of natto "goo"! (4)

Summary: We found that our NYrture natto contained at least ~15 million live Bacillus subtilis bacteria and ~1.5 million viable Bacillus subtilis spores per milliliter.  In eating terms, a generous serving of 50mls contains 750 million cells and 75 million viable spores of probiotic bacteria!  Noting that these results came from a month-old jar of our natto, the numbers may be even higher for a fresher batch! Truly, we could not be more pleased with these results, and we're certain that our fresh natto contains far more viable probiotic bacteria than any other (frozen) natto available here! 

References: (1)  http://micro.med.harvard.edu/faculty/rudnergraphic-lg.jpg  (2) Wikipedia [spore]: https://en.wikipedia.org/wiki/Spore. (3) Cowan, A. E. et al. PNAS.101:20, 7733-8.  (4) personal communication from Dr. Alex Meeske, Rudner lab, Harvard Medical School, now Maraffini lab, Rockefeller University.

Gaga for Goo: How probiotic is NYrture New York Natto?

As you see here, after fermentation NYrture New York Natto is held together by an impressive matrix of "neba-neba" (in Japanese), "goo" (in American) or "biofilm" (in scientific terms). This "goo" which gives natto such a unique and unfamiliar (to the Western palate) texture is highly prized and regarded as an indicator of natto quality in Japan.

This stickiness is a substance secreted by the probiotic Bacillus subtilis during natto fermentation which help to protect them from environmental stress and connects them together, allowing communication and physical contact through a common medium. This "goo", primarily a polymer of glutamatic acid (a natural protein amino acid) (1), helps the bacteria to form a stable community as an continuous layer of cells known as a "biofilm"(2). 

Ann Yonetani of NYrture New York Natto @ HMS

Ann Yonetani of NYrture New York Natto @ HMS

We wanted to find out "how probiotic is our natto?" or, in other words, "how many live bacteria" were really in there in this "goo"? So, we took our natto to labs at Harvard Medical School and got to do some real science looking at our food with some of the best tools and help available!

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So many "functional foods" on the market claim to be "probiotic"; yet it is unclear how many actual live bacteria remain in these foods by the time they reach consumers.  Our natto is made simply by fermenting GMO-free soybeans with a large innoculum of the healthy gut microbiome resident Bacillus subtilis, but we wanted to determine how much of these bacteria were still alive and well inside NYrture natto even after a few weeks of refrigerated aging (which improves its flavor, but what of its probiotic power)?  A simple question...

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which could also be answered quite simply by streaking out a sample of NYrture New York Natto "goo" on a sterile (LB) agar petri plate to see what, if anything, would grow…answer: LOTS!

Indeed, after two days at ambient temperature, robust growth of happy Bacillus subtilis originating from our natto!

Indeed, after two days at ambient temperature, robust growth of happy Bacillus subtilis originating from our natto!

Microscopy has always been my favorite scientific tool. We were so lucky to have the aid and goodwill of Bacillus subtilis scientists Paula Montero-Llopis, Alex Meeske and David Rudner at HMS Microbiology to expertly help us with imaging our "goo". We learned many interesting things….

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First and foremost, NYrture New York Natto is absolutely teeming with happy, living, dividing and sporulation Bacillus subtilis---even a full month after fermentation/production!

Phase image of "goo" diluted/rinsed in PBS, spotted onto agar pads & viewed on inverted epifluorescence scope. Live cells are dark with bright halos, surrounded by large amounts of fainter, fragmented bacterial cell debris. Fluorescence imaging …

Phase image of "goo" diluted/rinsed in PBS, spotted onto agar pads & viewed on inverted epifluorescence scope. Live cells are dark with bright halos, surrounded by large amounts of fainter, fragmented bacterial cell debris. Fluorescence imaging of DAPI staining of DNA (not shown here) confirms live/dead cells.

Rough approximation: 15 million live probiotic bacterial cells per milliliter are present in NYrture NY Natto after four weeks in the fridge (3)! A typical "serving" might be 50-100 times this volume. The numbers are huge, and undoubtedly would be much higher still in a fresher sample.  

Much more on this and other exciting findings from our lab experimentations to come….

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For now, many heartfelt thanks to our friends at Harvard Medical School, especially those in David Rudner and George Church's laboratories for their curiosity, assistance and enthusiasm to help take natto inquiry to a new level.  Stay tuned for more early results from Harvard and continuing studies at Columbia University!

Ann Yonetani back at the bench

Ann Yonetani back at the bench

References: (1) Bhat, A.R. et al. (2013) Bacillus subtilis natto: a non-toxic source of  poly-gamma-glutamic acid that could be used as a cryoprotectant for probiotic bacteria. AMB Express. 3:36. http://link.springer.com/article/10.1186%2F2191-0855-3-36(2) Wikipedia [biofilm]: https://en.wikipedia.org/wiki/Biofilm (3) personal communication, Alex Meeske, Harvard Medical School.